| Application | ESCORT™ IV is a liposomal formulation of a polycationic lipid and a neutral, non-transfecting lipid compound. ESCORT™ IV has been used successfully to transfect DNA into CHO-K1, COS-1 and NIH3T3 (in the presence of fetal bovine serum), primary keratinocytes, primary rat aortic smooth muscle, primary human lung alveolar cells, ECV304 (HUVEC), 293, C2/C12, and astrocytes. ESCORT™ IV is an excellent transfection reagent for Sf9 and T.ni insect cells resulting in higher efficiencies, better cell viability and requiring less transfection reagent and less DNA compared to other insect specific transfection reagents. ESCORT™ IV is recommended for the transfection of cultured cell lines. Peak activities were achieved using 4-6 μg ESCORT™ IV per μg of DNA on the tested cell lines grown in 35 mm dishes. No significant toxicity was observed at the concentrations used for optimal activity on the cell lines tested. |
